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ln order to investigate the effetcts of bisphenol A (BPA) on cell mediated immune reaction in vitro we examined the allogenic mixed lymphocyte reaction (MLR), splenocytes proliferation (SP) to T cell mitogens and IFN- γ production. Splenocytes of Balb/c mice (1.5×105 cells/well) were co-cultured with different numbers of mitomycin C-treated mature dentritic cells (DCs) in presence of BPA (25, 50, 100 ㎛) and (3H)thymidine incorporation (cpm) was measured by scintilation counting. Splenocytes (2×106 cells/well) were cultured with mitogens, Con A (2 ㎍/㎖), PHA (5 ㎍/㎖) and IL-2 (0.1 ㎍/㎖), or PMA (5 ng/㎖) and INO (1㎍/㎖) in presence of BPA (1, 10, 25, 50, 100 ㎛) and SP was assessed by MTT assay. IFN-γ levels in culture supernant were determined by ELISA. At low concentration, BPA slightly increased MLR, SP and IFN-γ levels, but at higher concentration it showed significant inhibitory effects on these immunological parameters. These results indicate that BPA is able to alternate cell-mediated immune reaction.
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